Translational_Unit

Part:BBa_K5078005:Design

Designed by: Jacob McFadden   Group: iGEM24_UVU-Utah-2   (2024-08-07)


pL1 nosZ P. stutzeri


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1356
    Illegal PstI site found at 3081
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2118
    Illegal PstI site found at 1356
    Illegal PstI site found at 3081
    Illegal NotI site found at 960
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2335
    Illegal BamHI site found at 1282
    Illegal XhoI site found at 849
    Illegal XhoI site found at 2403
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1356
    Illegal PstI site found at 3081
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1356
    Illegal PstI site found at 3081
    Illegal NgoMIV site found at 1890
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Place Holder


Source

These parts come from the Chlamy MoClo Kit.The PSAD promoter and PSAD terminator are derived from chlamydomonas reinhardtii. mCherry was derived from Discosoma sea anemones.

References